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بررسی آلودگی میکروبی و شیمیایی در میگوهای پرورشی و دریایی عرضه شده در شهرستان بندرعباس | ||
| تحقیقات آزمایشگاهی دامپزشکی | ||
| دوره 17، شماره 1 - شماره پیاپی 27، فروردین 1404، صفحه 127-136 اصل مقاله (480.79 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22075/jvlr.2025.37607.1165 | ||
| نویسندگان | ||
| سعید عسکری* 1؛ ابراهیم رحیمی2؛ سید مجید هاشمی3؛ امیر شاکریان2 | ||
| 1دانشگاه آزاد اسلامی واحد شهر کرد | ||
| 2استاد، گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران | ||
| 3استادیار، مرکز تحقیقات تغذیه و محصولات ارگانیک، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران | ||
| تاریخ دریافت: 11 اردیبهشت 1404، تاریخ بازنگری: 06 مرداد 1404، تاریخ پذیرش: 20 شهریور 1404 | ||
| چکیده | ||
| بهداشت و سلامت مواد غذایی دریایی یک نگرانی جهانی است. شیوع مقاومت ضد میکروبی و آلودگی به فلزات سنگین سبب پیامدهای ناگواری برای سلامت مصرفکنندگان میشود. هدف از پژوهش حاضر بررسی آلودگی به اشرشیاکلای و مقاومت آنتیبیوتیکی جدایهها و تعیین سرب و کادمیوم در میگوهای پرورشی و آزاد عرضهشده در بندرعباس است. 60 نمونه میگو پرورشی و دریایی از بازار بندرعباس در بهار تا پائیز 1403 نمونهگیری و به آزمایشگاه انتقال داده شد. از روش کشت خطی برای جداسازی اشرشیاکلای، تعیین مقاومت آنتیبیوتیک از روش Disk Diffusion و از دستگاه جذب اتمی برای تعیین فلزات سنگین استفاده شد. نتایج بررسی آلودگی میکروبی در میگوهای پرورشی و دریایی نشان داد از مجموع 60 نمونه میگو، 9 نمونه به اشرشیاکلای آلوده بود. به این ترتیب میزان آلودگی به اشرشیاکلای در میگوهای دریایی 7 نمونه (66/11 درصد) و میگوهای پرورشی 2 نمونه (33/3 درصد) بود و بیشترین مقاومت آنتیبیوتیکی مربوط به سولفامتاکسازول (100 درصد) و تتراسایکلین (88/88 درصد) و کمترین مقاومت مربوط به ایمیپنم (0) بود. نتایج ارزیابی فلزات سنگین نشان داد میزان آلودگی در میگوهای دریایی نسبت به سرب و کادمیوم فراتر از نمونههای پرورشی است. نتایج ما نشان داد که میگوهای دریایی و پرورشی از منابع آلودگی به اشرشیاکلای هستند و از لحاظ فلزات سنگین باید نسبت به آزادسازی فضلاب کارخانههای صنعتی و دیگر منابع آلودگی تصمیمات سختگیرانهای اتخاذ شود. | ||
| کلیدواژهها | ||
| میگو؛ اشرشیاکلای؛ مقاومت آنتیبیوتیکی؛ فلزات سنگین | ||
| عنوان مقاله [English] | ||
| Investigation of microbial and chemical contamination in farmed and marine shrimp supplied in Bandar Abbas county | ||
| نویسندگان [English] | ||
| saeed askari1؛ ٍEbrahim Rahimi2؛ Seyed Majid Hashemi3؛ Amir Shakerian2 | ||
| 1IsIamic Azad university of shahrekord | ||
| 2Professor, Food Hygiene Department, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| 3Assistant Professor, Nutrition and Organic Products Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| چکیده [English] | ||
| 127 The health and safety of seafood is a global concern. The prevalence of antimicrobial resistance and heavy metal contamination has adverse consequences for consumer health. The aim of the present study was to investigate Escherichia coli contamination and antibiotic resistance of isolates and to determine lead and cadmium in farmed and wild shrimp supplied in Bandar Abbas. 60 farmed and marine shrimp samples were sampled from the Bandar Abbas market From spring to autumn 2024 and transferred to the laboratory. The linear culture method was used to isolate Escherichia coli, the Disk Diffusion method was used to determine antibiotic resistance, and the atomic absorption spectrometer was used to determine heavy metals. The results of the investigation of microbial contamination in farmed and marine shrimp showed that out of a total of 60 shrimp samples, 9 samples were contaminated with Escherichia coli. Thus, the level of Escherichia coli contamination in marine shrimp was 7 samples (11.66%) and farmed shrimp was 2 samples (3.33%), and the highest antibiotic resistance was related to sulfamethoxazole (100%) and tetracycline (88.88%) and the lowest resistance was related to imipenem (0). The results of the heavy metal assessment showed that the level of contamination in marine shrimp with lead and cadmium was higher than in farmed samples. Our results showed that marine and farmed shrimp are sources of Escherichia coli contamination and in terms of heavy metals, strict decisions should be made regarding the release of industrial waste and other sources of contamination. | ||
| کلیدواژهها [English] | ||
| Shrimp, Escherichia coli, Antibiotic resistance, Heavy metals | ||
| مراجع | ||
|
Adler, G., Nędzarek, A. & Tórz, A. 2019. Concentrations of selected metals (Na, K, Ca, mg, FE, CU, Zn, al, Ni, PB, cd) in coffee. Slovenian Journal of Public Health, 58, 187-193. Ahmad, I., Malak, H. A. & Abulreesh, H. H. 2021. Environmental antimicrobial resistance and its drivers: a potential threat to public health. Journal of Global Antimicrobial Resistance, 27, 101-111. Ahmed, M. K., Baki, M. A., Islam, M. S., Kundu, G. K., Habibullah-Al-Mamun, M., Sarkar, S. K. & Hossain, M. M. 2015. Human health risk assessment of heavy metals in tropical fish and shellfish collected from the river Buriganga, Bangladesh. Environmental science and pollution research, 22, 15880-15890. Alhabib, I. & Elhadi, N. 2024. Antimicrobial resistance pattern of Escherichia coli isolated from imported frozen shrimp in Saudi Arabia. PeerJ, 12, e18689. Atwill, E. R. & Jeamsripong, S. 2021. Bacterial diversity and potential risk factors associated with Salmonella contamination of seafood products sold in retail markets in Bangkok, Thailand. PeerJ, 9, e12694. Baki, M. A., Hossain, M. M., Akter, J., Quraishi, S. B., Shojib, M. F. H., Ullah, A. A. & Khan, M. F. 2018. Concentration of heavy metals in seafood (fishes, shrimp, lobster and crabs) and human health assessment in Saint Martin Island, Bangladesh. Ecotoxicology and environmental safety, 159, 153-163. Bantawa, K., Sah, S. N., Subba Limbu, D., Subba, P. & Ghimire, A. 2019. Antibiotic resistance patterns of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella and Vibrio isolated from chicken, pork, buffalo and goat meat in eastern Nepal. BMC research notes, 12, 1-6. Bharti, R. & Sharma, R. 2022. Effect of heavy metals: An overview. Materials Today: Proceedings, 51, 880-885. Chakravarty, M. S., Ganesh, P., Amaranth, D., Shanthi Sudha, B. & Subhashini, M. 2015. Escherichia coli-occurrence in the meat of shrimp, fish, chicken and mutton and its antibiotic resistance. European Journal of Experimental Biology, 5, 41-48. Commission, C.A. (1995). Codex stan 193-1995. Codex general standard for contaminants and toxins in food and feed. 1-77. Available at: https://www.fao.org/fao-who-codexalimentarius/en/ Ec. (2008). Commission Regulation (EC) No 1881- 2006-"Setting maximum levels for criteria contaminations in foodstuffs". European Commission, Commission Regulation (EC) No.466/2001 SettingMaximum Levels for Certain Contaminants in Foodstuffs. https://eur-lex.europa.eu Changkaew, K., Utrarachkij, F., Siripanichgon, K., Nakajima, C., Suthienkul, O. & Suzuki, Y. 2014. Characterization of antibiotic resistance in Escherichia coli isolated from shrimps and their environment. Journal of food protection, 77, 1394-1401. Cheng, H., Jiang, H., Fang, J. & Zhu, C. 2019. Antibiotic resistance and characteristics of integrons in Escherichia coli isolated from Penaeus vannamei at a freshwater shrimp farm in Zhejiang Province, China. Journal of food protection, 82, 470-478. Copat, C., Bella, F., Castaing, M., Fallico, R., Sciacca, S. & Ferrante, M. 2012. Heavy metals concentrations in fish from Sicily (Mediterranean Sea) and evaluation of possible health risks to consumers. Bulletin of Environmental Contamination and Toxicology, 88, 78-83. Costa, R. A. 2013. Escherichia coli in seafood: A brief overview. Dewi, R. R., Hassan, L., Daud, H. M., Matori, M. F., Nordin, F., Ahmad, N. I. & Zakaria, Z. 2022. Prevalence and antimicrobial resistance of Escherichia coli, Salmonella and Vibrio derived from farm-raised red hybrid tilapia (Oreochromis spp.) and Asian sea bass (Lates calcarifer, Bloch 1970) on the West Coast of Peninsular Malaysia. Antibiotics, 11, 136. Dutta, C. & Sengupta, C. 2016. Prevalence of Escherichia coli in Fish and Shrimps obtained from retail Fish markets in and around Kolkata, India. Frontiers in Environmental Microbiology, 2, 1-5. Fsanza. (2010). Food Standards Australia and New Zealand Standard 1.4.1 (FSANZS), Contaminants, and Natural Toxicants laid down maximum levels of five heavy metals in specified food. Available at: https://www.foodstandards.gov.au Heidarzadi, M. A., Rahnama, M., Alipoureskandani, M., Saadati, D. & Afsharimoghadam, A. 2021. Salmonella and Escherichia coli contamination in samosas presented in Sistan and Baluchestan province and antibiotic resistance of isolates. Food Hygiene, 11, 81-90. Ireland, F.S.A.O. (2009). Food Safety Authority of Ireland.Mercury, Lead, Cadmium, Tin, and Arsenic in Food, Food Safety Authority of Ireland. Available at: https://www.fsai.ie. Institute of Standards and Industrial Research of Iran (ISIRI), (2009). NO 12968. Food and Feed Maximum limit of heavy metals and test methods. (In Persian) Jeamsripong, S., Thaotumpitak, V., Anuntawirun, S., Roongrojmongkhon, N., Atwill, E. R. & Hinthong, W. 2022. Molecular epidemiology of antimicrobial resistance and virulence profiles of Escherichia coli, Salmonella spp., and Vibrio spp. isolated from coastal seawater for aquaculture. Antibiotics, 11, 1688. Kam, K. Y., Jaafar, M. F., Sani, M. H., Suhaimi, M. S. & Mohd Zain, N. A. 2022. Prevalence of Antibiotic Resistance Bacteria in Aquaculture Sources in Johor, Malaysia. Journal of Tropical Life Science, 12. Khodabakhshi, A., Sedehi, M. & Shakeri, K. 2016. Investigation of heavy metals in edible mushrooms consumed in Shahrekord. Journal of Shahrekord University of Medical Sciences, 18, 54-62. Lee, K.-G., Kweon, H., Yeo, J.-H., Woo, S., Han, S. & Kim, J.-H. 2011. Characterization of tyrosine-rich Antheraea pernyi silk fibroin hydrolysate. International Journal of Biological Macromolecules, 48, 223-226. Link, D. T., Viana, G. G. F., Siqueira, L. P., Ferraz, C. M., Rodrigues, R. A., Mathias, L. A., Cardozo, M. V. & Rossi, G. A. M. 2024. Assessing the Microbial Quality of Shrimp (Xiphonaeus kroyeri) and Mussels (Perna perna) Illegally Sold in the Vitória Region, Brazil, and Investigating the Antimicrobial Resistance of Escherichia coli Isolates. Antibiotics, 13, 242. Mattia, G. D., Bravi, M. C., Laurenti, O., Luca, O. D., Palmeri, A., Sabatucci, A., Mendico, G. & Ghiselli, A. 2004. Impairment of cell and plasma redox state in subjects professionally exposed to chromium. American journal of industrial medicine, 46, 120-125. Narimisa, M. & Rahimi, E. 2023. Determining the microbial and chemical characteristics of shrimps supplied in Abadan city. National Standard of the People’s Republic of China (NSPRC). (2011). National Food Safety Standard, Issued by the Ministry of Health of the People’s Republic of China.1-14. Pires, D., De Morais, A., Coelho, C., Marinho, A., Góes, L., Augusta, I., Ferreira, F. & Saldanha, T. 2018. Nutritional composition, fatty acids and cholesterol levels in Atlantic white shrimp (Litopenaeus schimitti). International Food Research Journal, 25, 151-157. Sarkar, T., Alam, M. M., Parvin, N., Fardous, Z., Chowdhury, A. Z., Hossain, S., Haque, M. & Biswas, N. 2016. Assessment of heavy metals contamination and human health risk in shrimp collected from different farms and rivers at Khulna-Satkhira region, Bangladesh. Toxicology reports, 3, 346-350. Sivaraman, G. K., Rajan, V., Vijayan, A., Elangovan, R., Prendiville, A. & Bachmann, T. T. 2021. Antibiotic resistance profiles and molecular characteristics of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae isolated from shrimp aquaculture farms in Kerala, India. Frontiers in Microbiology, 12, 622891. Sukmawaty, E., Nur, F. & Suriani, S. 2021. Bacterial Contamination at Whiteleg Shrimp (Litopeanaeus vannamei) in Aquaculture. Jurnal Biodjati, 6, 136-145. Sultana, S., Hossain, M. B., Choudhury, T. R., Yu, J., Rana, M. S., Noman, M. A., Hosen, M. M., Paray, B. A. & Arai, T. 2022. Ecological and human health risk assessment of heavy metals in cultured shrimp and aquaculture sludge. Toxics, 10, 175. Talukder, B., Chakma, F., Sabuj, A. A. M., Haque, Z. F., Rahman, M. T. & Saha, S. 2019. Identification, prevalence and antimicrobial susceptibility of major pathogenic bacteria in exportable shrimp (Penaeus monodon) of southern Bangladesh. Int J Fish Aquat Stud, 7, 96-9. Tchounwou, P. B., Yedjou, C. G., Patlolla, A. K. & Sutton, D. J. 2012. Heavy metal toxicity and the environment. Molecular, clinical and environmental toxicology: volume 3: environmental toxicology, 133-164. Zhang, S., Huang, Y., Yang, G., Wu, Q., Zhang, J., Wang, J., Ding, Y., Su, Y., Ye, Q. & Wu, S. 2024. High prevalence of multidrug-resistant Escherichia coli in retail aquatic products in China and the first report of mcr-1-positive extended-spectrum β-lactamase-producing E. coli ST2705 and ST10 in fish. International Journal of Food Microbiology, 408, 110449. | ||
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