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ارزیابی قدرت جذب وجداسازی آمینو اسیدها (Arg.و .Glu) توسط اگزروژل تترااتوکسی اورتو‌سیلان | ||
| شیمى کاربردى روز | ||
| دوره 18، شماره 68، مهر 1402، صفحه 47-62 اصل مقاله (1.11 M) | ||
| نوع مقاله: مقاله علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22075/chem.2022.27441.2081 | ||
| نویسندگان | ||
| علی اصل روستا؛ حسن حسنی* ؛ علیرضا اکبری؛ نورالله فیضی | ||
| گروه شیمی، دانشکده علوم، دانشگاه پیام نور تهران، تهران، ایران | ||
| تاریخ دریافت: 11 تیر 1401، تاریخ بازنگری: 17 آبان 1401، تاریخ پذیرش: 26 آذر 1401 | ||
| چکیده | ||
| در این تحقیق توانایی اگزروژل تترا اتوکسی اورتو سیلان (TEOS) به عنوان جاذبی ارزان، ایمن با ساختار قوی مکانیکی در جداسازی آمینو اسیدهای اسیدی( .Glu) و بازی( Arg.) در ستون کروماتوگرافی جذبی مورد مطالعه قرار گرفت.ابتدا اثر pHو سپس تاثیر میزان آب گریزی سطح بستر جاذب و همچنین طول ستون کروماتوگرافی بر روی میزان وسرعت تخلیص مورد ارزیابی قرار گرفت.با توجه به نتایج بدست آمده ،در pH خنثی(7) میزان تفکیک دو نمونه آمینو اسید مورد آزمایش بهتر صورت گرفت. در ادامه با تهیه مشتقات اورموسیل دار جاذب اگزروژل (TEOS + Me، TEOS + Et و TEOS + Pro) و آزمایش بر روی نمونه آمینو اسیدهای فوق الذکر ،مشخص گردید که تاثیر هیدروفوبیسیتی سطح بستر در مقایسه با تاثیر عامل pH قابل ملاحظه نیست .افزایش طول ستون کروماتوگرافی بر تفکیک و جداسازی بهتر نمونه های فوق موثر بود. | ||
| کلیدواژه‌ها | ||
| اگزروژل تترااتوکسی اورتو‌سیلان (TEOS)؛ اورموسیل؛ آمینو اسید؛ جذب | ||
| عنوان مقاله [English] | ||
| Studying the process of absorption and separation of amino acids (Arg and Glu) by tetraethoxyortho-silane exogel adsorbent | ||
| نویسندگان [English] | ||
| Ali Asl Rousta؛ hassan hassani؛ Alireza Akbari؛ Noroallah Feizi | ||
| department of chemistry Payam noor university Tehran, Iran | ||
| چکیده [English] | ||
| In this research, the ability of tetraethoxyorthosilane (TEOS) exogel as a cheap, safe adsorbent with a strong mechanical structure was studied in the separation of acidic (.Glu) and basic (Arg.) amino acids in an absorption chromatography column. First, the effect of pH and then The effect of the length of the chromatography column as well as the hydrophobicity factor of the adsorbent surface on the amount and speed of purification was evaluated. According to the results, the separation rate of the two tested amino acid samples was better at neutral pH (7). In the following, by preparing ormucil derivatives of exogel absorbent (TEOS + Me, TEOS + Et and TEOS + Pro) and testing on the aforementioned amino acids sample, it was found that the effect of the hydrophobicity of the substrate surface is not significant compared to the effect of the pH factor. The length of the chromatography column was effective on the better separation of the above samples. | ||
| کلیدواژه‌ها [English] | ||
| Tetraethoxyorthosilane exogel (TEOS), Ormusil, amino acid, absorption | ||
| مراجع | ||
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