پیوندهای مفید
آمار
| تعداد نشریات | 21 |
| تعداد شمارهها | 687 |
| تعداد مقالات | 9,985 |
| تعداد مشاهده مقاله | 70,957,064 |
| تعداد دریافت فایل اصل مقاله | 62,422,381 |
تعیین آلودگی به لیستریا مونوسیتوژنز و بررسی فراوانی ژنهای حدت و مقاومت آنتیبیوتیکی در مواد غذایی آماده مصرف | ||
| تحقیقات آزمایشگاهی دامپزشکی | ||
| دوره 17، شماره 1 - شماره پیاپی 27، فروردین 1404، صفحه 51-61 اصل مقاله (521.77 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22075/jvlr.2025.37325.1160 | ||
| نویسندگان | ||
| نعمت اله مهدی زاده قهدریجانی* 1؛ سید مجید هاشمی2 | ||
| 1گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران | ||
| 2استادیار، مرکز تحقیقات تغذیه و محصولات ارگانیک، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران | ||
| تاریخ دریافت: 17 فروردین 1404، تاریخ بازنگری: 19 اردیبهشت 1404، تاریخ پذیرش: 24 خرداد 1404 | ||
| چکیده | ||
| لیستریا مونوسیتوژنز یک پاتوژن خطرناک منتقل شونده از طریق مواد غذایی است که باعث ایجاد لیستریوز برای افراد دارای نقص ایمنی، افراد مسن و زنان باردار میشود و در کنار مقاومت ضد میکروبی (AMR) به یک مسئله جدی بهداشت عمومی تبدیل شده است. تمرکز این پژوهش روی تعیین آلودگی به مونوسیتوژنز و بررسی فراوانی ژنهای حدت و مقاومت آنتیبیوتیکی در مواد غذایی آماده است. در این مطالعه تعداد 180 نمونه مواد غذایی شامل سالاد اولویه، ناگت مرغ، ناگت گوشت، ساندویچ مرغ، ساندویچ کالباس و شنیسل مرغ به صورت تصادفی از مراکز عرضه در شهرستان فلاورجان استان اصفهان نمونهگیری و به آزمایشگاه انتقال داده شد. نتایج نشان داد میزان شیوع آلودگی به لیستریا مونوسیتوژنز 13 نمونه (22/7 درصد) بود و بیشترین فراوانی ژنهای مقاومت آنتی بیوتیکی مربوط به ژن strA 100 درصد و ermA 61/84 درصد بود. شیوع ژنهای ermB، tetS، tetA و ermC در سویه لیستریا مونوسیتوژنز مورد مطالعه به ترتیب 46/38 درصد، 15/46 درصد، صفر و صفر بود. نتایج PCR نشان داد از 13 نمونه مواد غذایی آلوده به لیستریا مونوسیتوژنز 2 نمونه (11/1 درصد) مربوط به ژن inlA بود. نتایج آنتیبیوتیکی نشان داد بیشترین مقاومت مربوط به پنیسیلین و آمپیسیلین با 100 درصد مقاومت و کمترین مقاومت مربوط به کلرامفنیکل و ایمیپنم با 100 درصد حساسیت بود. نتایج نشان داد مواد غذایی RTE میتوانند حامل لیستریا مونوسیتوژنز باشند که در صورت بروز عفونتهای ناشی از آن استفاده از آنتیبیوتیکها به صورت خودسرانه محدود گردد. | ||
| کلیدواژهها | ||
| لیستریا مونوسیتوژنز؛ مقاومت آنتیبیوتیکی؛ مقاومت ضد میکروبی؛ مواد غذایی آماده | ||
| عنوان مقاله [English] | ||
| Determination of Listeria monocytogenes contamination and investigation of the frequency of virulence and antibiotic resistance genes in ready-to-eat foods | ||
| نویسندگان [English] | ||
| Nematollah Mehdizadeh Ghahderijani1؛ Seyed Majid Hashemi2 | ||
| 1Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| 2Assistant Professor, Nutrition and Organic Products Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| چکیده [English] | ||
| 51 Listeria monocytogenes is a dangerous foodborne pathogen that causes listeriosis in immunocompromised individuals, the elderly, and pregnant women, and has become a serious public health issue along with antimicrobial resistance (AMR). The focus of this study is on determining Listeria monocytogenes contamination and investigation of the frequency of virulence and antibiotic resistance genes in ready-to-eat foods. In this study, 180 food samples including salad dressing, chicken nuggets, meat nuggets, chicken sandwiches, sausage sandwiches, and chicken schnitzel were randomly sampled from distribution centers in Falavarjan County, Isfahan Province and transported to the laboratory. The results showed that the prevalence of Listeria monocytogenes infection was 13 samples (7.22%) and the highest frequency of antibiotic resistance genes was related to the strA gene (100%) and ermA (84.61%). The prevalence of ermB, tetS, tetA and ermC genes in the studied Listeria monocytogenes strain was 38.46%, 46.15%, zero and zero, respectively. PCR results showed that out of 13 food samples contaminated with Listeria monocytogenes, 2 samples (1.11%) were related to the inlA gene. Antibiotic results showed that the highest resistance was related to penicillin and ampicillin with 100% resistance and the lowest resistance was related to chloramphenicol and imipenem with 100% sensitivity. The results showed that RTE foods can carry Listeria monocytogenes, which in the event of infections caused by it, the use of antibiotics should be arbitrarily limited. | ||
| کلیدواژهها [English] | ||
| Listeria monocytogenes, antibiotic resistance, antimicrobial resistance, ready-to-eat foods | ||
| مراجع | ||
|
Abatcha, M. )2017(. Salmonella and Listeria monocytogenes: A review of prevalence and antibiotic resistance in chickens and their processing environ-ments. Advances in Animal and Veterinary Sciences, 5, 395-403. Almualla, N. A., Laleye, L. C., Abushelaibi, A. A., Al-Qassemi, R. A., Wasesa, A. A., Baboucarr, J. (2010). Aspects of the microbiological quality and safety of ready-to-eat foods in Sharjah supermarkets in the United Arab Emirates. Journal of food protection, 73, 1328-1331. Ardlan, S. & Özdemir, F. (2020). Prevalence and antimicrobial resistance of Listeria species and molecular characterization of Listeria monocytogenes isolated from retail ready-to-eat foods. FEMS Microbiology Letters, 367, fnaa006. Baquero, F., F. Lanza, V., Duval, M., Coque, T. M. (2020). Ecogenetics of antibiotic resistance in Listeria monocytogenes. Molecular microbiology, 113, 570-579. Bonanno, L., Bergis, H., Gnanou-Besse, N., Asséré, A., Danan, C. (2024). Which domestic refrigerator temperatures in Europe?-Focus on shelf-life studies regarding Listeria monocytogenes (Lm) in ready-to-eat (RTE) foods. Food Microbiology, 123, 104595. Bustamante, F., Maury-Sintjago, E., Leal, F. C., Acuña, S., Aguirre, J., Troncoso, M., Parra-Flores, J. (2020). Presence of Listeria monocytogenes in ready-to-eat artisanal Chilean foods. Microorganisms, 8, 1669. Caruso, M., Fraccalvieri, R., Pasquali, F., Santagada, G., Latorre, L. M., Difato, L. M., Parisi, A. (2020). Antimicrobial susceptibility and multilocus sequence typing of Listeria monocytogenes isolated over 11 years from food, humans, and the environment in Italy. Foodborne pathogens and disease, 17, 284-294. Ebadifar, A. M., Jaydari, A., Shams, N., Rahimi, H. (2022). Prevalence of Listeria monocytogenes in raw milk of the healthy cattle in Lorestan Province (Iran) by PCR. Journal of Advanced Biomedical Sciences. ESFA 2015. The European Union Summary Report. Trends and Sources of Zoonoses, Zoonotic Agent and Food-borne Outbreaks in 2015. EFSA Journal, 14. Farhoumand, P., Hassanzadazar, H., Soltanpour, M. S., Aminzare, M., & Abbasi, Z. (2020). Prevalence, genotyping and antibiotic resistance of Listeria monocytogenes and Escherichia coli in fresh beef and chicken meats marketed in Zanjan, Iran. Iranian Journal of Microbiology, 12(6), 537. Hailu, W., Helmy, Y. A., Carney-Knisely, G., Kauffman, M., Fraga, D., Rajashekara, G. (2021). Prevalence and antimicrobial resistance profiles of foodborne pathogens isolated from dairy cattle and poultry manure amended farms in northeastern Ohio, the United States. Antibiotics, 10, 1450. Hanes, R. M. Huang, Z. (2022). Investigation of Antimicrobial Resistance Genes in Listeria monocytogenes from 2010 through to 2021. International Journal of Environmental Research and Public Health, 19, 5506. Heidarlo, M. N., Lotfollahi, L., Yousefi, S., Lohrasbi, V., Irajian, G., Talebi, M. (2021). Analysis of virulence genes and molecular typing of Listeria monocytogenes isolates from human, food, and livestock from 2008 to 2016 in Iran. Tropical Animal Health and Production, 53, 1-9. Heidarzadi, M., Rahnama, M., Alipoureskandani, M., Saadati, D. Afsharimoqadam, A. (2021). Salmonella and Escherichia coli contamination in samosas presented in Sistan and Baluchestan province and antibiotic resistance of isolates. Food Hygiene, 11, 42. Kawacka, I. Olejnik-Schmidt, A. (2024). High Prevalence of Virulence-Associated Genes and Length Polymorphism in actA and inlB Genes Identified in Listeria monocytogenes Isolates from Meat Products and Meat-Processing Environments in Poland. Pathogens, 13, 444. Koskar, J., Kramarenko, T., Meremäe, K., Kuningas, M., Sogel, J., Mäesaar, M., Roasto, M. (2019). Prevalence and numbers of Listeria monocytogenes in various ready-to-eat foods over a 5-year period in Estonia. Journal of food protection, 82, 597-604. Kotzekidou, P. (2013). Microbiological examination of ready-to-eat foods and ready-to-bake frozen pastries from university canteens. Food Microbiology, 34, 337-343. McLauchlin, J., Aird, H., Amar, C. F. L., Jenkins, C., Jørgensen, F., Lai, S., Willis, C. (2022). Microbiological Quality of Ready-to-Eat Salad Products Collected from Retail and Catering Settings in England during 2020 to 2021. Journal of Food Protection, 85, 1680-1689. Mureddu, A., Mazza, R., Fois, F., Meloni, D., Bacciu, R., Piras, F., Mazzette, R. (2014). Listeria monocytogenes persistence in ready-to-eat sausages and in processing plants. Italian Journal of Food Safety, 3. Nosheena, A. S., Naeem, M., Jamil, H., Farooq, P., Sadiq, M. B., Tariq, H., & Akbar, A. (2022). Presence of listeria species in fresh salad products collected from quetta pakistan. Osaili, T. M., Alaboudi, A. R., Nesiar, E. A. (2011). Prevalence of Listeria spp. and antibiotic susceptibility of Listeria monocytogenes isolated from raw chicken and ready-to-eat chicken products in Jordan. Food control, 22, 586-590. Parra-Flores, J., Holý, O., Bustamante, F., Lepuschitz, S., Pietzka, A., Contreras-Fernández, A., Ruppitsch, W. (2022). Virulence and antibiotic resistance genes in Listeria monocytogenes strains isolated from ready-to-eat foods in Chile. Frontiers in Microbiology, 12, 796040. Pizarro J. Cossart, P. (2018). Listeria monocytogenes: cell biology of invasion and intracellular growth. Microbiology spectrum, 6, 10.1128/microbiolspec. gpp3-0013-2018. Povolyaeva, O., Chalenko, Y., Kalinin, E., Kolbasova, O., Pivova, E., Kolbasov, D., Ermolaeva, S. (2020). Listeria monocytogenes infection of bat Pipistrellus nathusii epithelial cells depends on the invasion factors InlA and InlB. Pathogens, 9, 867. Rahimi, E., Heidarzadi, M. Vahed, N. (2023). Investigation of the prevalence of Listeria monocytogenes and Salmonella in fish, shrimp and eggs presented in Shahrekord city, Iran. Utilization and Cultivation of Aquatics, 12, 145-156. Şanlıbaba, P., Tezel, B. U., Çakmak, G. A. (2018). Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready‐to‐Eat Foods in Turkey. Journal of Food Quality, 2018. 7693782. Walsh, D., Duffy, G., Sheridan, J. J., Blair, I. S., McDowell, D. A. (2001). Antibiotic resistance among Listeria, including Listeria monocytogenes, in retail foods. Journal of Applied Microbiology, 90, 517-522. Wong, W. C., Pui, C. F., Tunung, R., Cheah, Y. K., Nakaguchi, Y., Nishibuchi, M., Son, R. (2012). Prevalence of Listeria monocytogenes in frozen burger patties in Malaysia. International Food Research Journal, 19. Z amuz, S., Munekata, P. E., Dzuvor, C. K., Zhang, W., Sant'Ana, A. S., & Lorenzo, J. M. (2021). The role of phenolic compounds against Listeria monocytogenes in food. A review. Trends in Food Science & Technology, 110, 385-392. Zhang, P., Ji, L., Wu, X., Chen, L., Yan, W., & Dong, F. (2024). Prevalence, Genotypic Characteristics, and Antibiotic Resistance of Listeria monocytogenes From Retail Foods in Huzhou, China. Journal of Food Protection, 87, 100307.
| ||
|
آمار تعداد مشاهده مقاله: 214 تعداد دریافت فایل اصل مقاله: 219 |
||