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ردیابی مولکولی اعضاء جنس بورلیا در نمونه خون اردک با استفاده از روش nested-PCR | ||
| تحقیقات آزمایشگاهی دامپزشکی | ||
| دوره 17، شماره 1 - شماره پیاپی 27، فروردین 1404، صفحه 63-70 اصل مقاله (438.26 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22075/jvlr.2025.34405.1114 | ||
| نویسندگان | ||
| نسترن علیزاده1؛ حمید استاجی* 2 | ||
| 1گروه پاتوبیولوژی دانشکده دامپزشکی دانشگاه سمنان | ||
| 2دانشکده دامپزشکی دانشگاه سمنان | ||
| تاریخ دریافت: 21 خرداد 1403، تاریخ بازنگری: 11 تیر 1404، تاریخ پذیرش: 11 خرداد 1404 | ||
| چکیده | ||
| پرندگان یکی از مهم ترین مخزنهای بورلیا و یکی از مهمترین عوامل انتقال آلودگی به انسان هستند. از اینرو هدف از انجام مطالعه حاضر ردیابی مولکولی اعضاء جنس بورلیا در نمونه خون اردک با استفاده از روش Nested-PCR بود. در این مطالعه از ۲۰۰ اردک بومی منطقه مازندران نمونه خون جمع اوری و در زمان جمع اوری نمونه خون، اطلاعات پرنده شامل جنس وسن نیز یادداشت شد. خون گیری از ورید برکیال بال پرنده انجام شد و در لوله های حاوی EDTA در کنار یخ به آزمایشگاه منتقل شد و پس از گسترش خونی و بررسی لامها با استفاده از تکنیک PCR و استفاده از پرایمرهای اختصاصی میزان آلودگی به جنس بورلیا در خون پرندگان مورد مطالعه بررسی شد . در این مطالعه در مجموع ۲۰۰. نمونه خون اردک مورد بررسی مولکولی قرار گرفت. برای تشخیص گونه های بورلیا از روش Nested-PCR استفاده شد. تجزیه و تحلیل اولیه نتایج نشان داد که از۲۰۰ اردک آزمایش شده، ۲اردک برای آلودگی با بورلیا مثبت بود. نتایج این مطالعه حاکی از شیوع این بیماری در اردکهای استان مازندران است و بایستی جهت جلوگیری از انتقال بیماری به فارمهای منطقه و همچنین انسان، با رعایت اصول بهداشتی را مد نظر قرار داد. | ||
| کلیدواژهها | ||
| بورلیا؛ اردک؛ Nested-PCR | ||
| عنوان مقاله [English] | ||
| Molecular detection of members of Borrelia genus in duck blood sample using nested-PCR method | ||
| نویسندگان [English] | ||
| Nastaran Alizadeh1؛ Hamid Staji2 | ||
| 1Departement of Pathobiology, faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| 2Semnan university | ||
| چکیده [English] | ||
| Birds are among the most important reservoirs of Borrelia and play a key role in the transmission of infection to humans. Therefore, the aim of the present study was the molecular detection of Borrelia species in duck blood samples using the nested PCR method. In this study, blood samples were collected from 200 native ducks in the Mazandaran region. At the time of sampling, information such as the gender and age of each bird was recorded. Blood was drawn from the brachial vein of the wing and collected in EDTA-containing tubes. Following blood smear preparation and slide examination, molecular analysis was performed using the PCR technique with specific primers to detect the presence of Borrelia infection in the sampled birds. In total, 200 duck samples were subjected to molecular examination. Nested-PCR was employed for the detection of Borrelia species. Both forward and reverse primers were used for species identification and, potentially, for gender differentiation of the pathogen. Preliminary nested-PCR analysis revealed that 2 out of the 200 ducks tested positive for Borrelia infection. Initially, 11 out of 36 microtubes containing samples tested positive in the nested-PCR machine, but subsequent confirmation tests narrowed the number of positive cases to 2. The results of this study highlight the presence of Borrelia infection in ducks from the Mazandaran province. These findings should be taken into account for biosecurity planning, especially to prevent disease transmission to local farms and humans, by observing appropriate public health and veterinary measures. | ||
| کلیدواژهها [English] | ||
| Borrelia, duck, nested-PCR | ||
| مراجع | ||
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