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بررسی شیوع و حساسیت آنتیبیوتیکی بروسلا ملیتنسیس در لبنیات عرضهشده در شهرستان گرمسار | ||
| تحقیقات آزمایشگاهی دامپزشکی | ||
| دوره 16، شماره 2 - شماره پیاپی 26، بهمن 1403، صفحه 131-141 اصل مقاله (505.55 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22075/jvlr.2025.35717.1134 | ||
| نویسندگان | ||
| نگین ایجی* 1؛ ابراهیم رحیمی2 | ||
| 1دانشگاه آزاد شهرکرد ،دانشکده دامپزشکی،گروه بهداشت کنترل کیفی مواد غذایی | ||
| 2استاد، گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران | ||
| تاریخ دریافت: 02 آبان 1403، تاریخ بازنگری: 30 آذر 1403، تاریخ پذیرش: 30 دی 1403 | ||
| چکیده | ||
| شیر خام آلوده به انواع بروسلا از مهمترین راههای انتقال بروسلوز به انسان است. هدف اصلی از مطالعه حاضر بررسی شیوع و حساسیت آنتیبیوتیکی بروسلا ملیتنسیس در لبنیات عرضهشده در شهرستان گرمسار است. در این پژوهش 180 نمونه لبنیات شامل شیر خام گاو، گوسفند، بز، گاومیش، پنیر سنتی و ماست سنتی عرضهشده در شهرستان گرمسار نمونهگیری و در شرایط سرما به آزمایشگاه انتقال داده شد. نمونهها توسط تست MRT، کشتخطی و Multiplex PCR ردیابی شده و به روش انتشار دیسک کربی-بائر مقاومت آنتیبیوتیکی آنها تعیین شد. نتایج نشان داد روش MRT میزان آلودگی بروسلا ملیتنسیس 11/11 درصد، کشت خطی 22/22 درصد و PCR 78/7 درصد تعیین شد. فراوانی ژنهای حدت bvfA 9 نمونه (25/64 درصد)، virfB 5 نمونه (74/35 درصد)، ure 3 نمونه (42/21) بود. مقاومت آنتیبیوتیکی نشان داد بیشترین مقاومت مربوط به تتراسایکلین (5/92 درصد) و آمپیسیلین (5/82 درصد) و کمترین مقاومت برای ریفامپین و ایمیپنم (0 درصد) بود. با توجه به نتایج به دست آمده میتوان نتیجهگرفت مصرف لبنیات خام و سنتی میتواند منبع انتقال بروسلوز به انسان باشد و باید استفاده از لبنیات سنتی و غیرپاستوریزه ممنوع گردد و حساسیتها نسبت به وضع قوانین سختگیرانه نسبت به مراکز عرضه بیشتر شود. | ||
| کلیدواژه‌ها | ||
| تب مالت؛ بروسلا ملیتنسیس؛ ژن حدت؛ مقاومت آنتیبیوتیکی؛ لبنیات | ||
| عنوان مقاله [English] | ||
| Investigation of the prevalence and antibiotic susceptibility of Brucella melitensis in dairy products supplied in Garmsar County | ||
| نویسندگان [English] | ||
| Negin Eiji1؛ Ebrahim Rahimi2 | ||
| 1Shahrekord Azad University, Faculty of Veterinary Medicine, Department of Food Quality Control Health | ||
| 2Professor, Food Hygiene Department, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran | ||
| چکیده [English] | ||
| Raw milk contaminated with Brucella species is one of the most important ways of transmitting brucellosis to humans. The aim of the present study was to investigate the prevalence and antibiotic susceptibility of Brucella melitensis in dairy products sold in Garmsar city. In this study, 180 dairy samples including raw Milk cow, sheep, goat and buffalo, traditional cheese, and traditional yogurt sold in Garmsar city were sampled and transferred to the laboratory under cold conditions. The samples were detected by MRT test, streak culture, and Multiplex PCR, and their antibiotic resistance was determined by the Kirby-Bauer disk diffusion method. The results showed that the MRT method determined the infection rate of Brucella melitensis to be 11.11%, streak culture to be 22.22%, and PCR to be 7.78%. The frequency of virulence genes was bvfA in 9 samples (64.25%), virfB in 5 samples (35.74%), and ure in 3 samples (21.42%). Antibiotic resistance showed that the highest resistance was to tetracycline (92.5%) and ampicillin (82.5%) and the lowest resistance was to rifampin and imipenem (0%). According to the results obtained, it can be concluded that the consumption of raw and traditional dairy products can be a source of transmission of malt fever to humans and the use of traditional and unpasteurized dairy products should be prohibited. | ||
| کلیدواژه‌ها [English] | ||
| Malt fever, Brucella melitensis, virulence gene, antibiotic resistance, dairy | ||
| مراجع | ||
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